Real-Time-PCR Assay for Diagnosis of Entamoeba histolytica Infection
نویسندگان
چکیده
منابع مشابه
Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples.
A closed-tube, real-time PCR assay was developed for sensitive and specific detection and differentiation of the two closely related intestinal protozoan parasites Entamoeba histolytica and Entamoeba dispar directly from human feces. The assay is performed with the LightCycler system using fluorescence-labeled detection probes and primers amplifying a 310-bp fragment from the high-copy-number, ...
متن کاملA real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient
Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...
متن کاملFreezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.
PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.
متن کاملComparison of PCR, isoenzyme analysis, and antigen detection for diagnosis of Entamoeba histolytica infection.
The diagnosis of amebiasis by microscopic identification of the parasite in stool is insensitive and unable to distinguish the invasive parasite Entamoeba histolytica from the commensal parasite E. dispar. In this study, we have tested a PCR technique for the detection of E. histolytica and compared it with isoenzyme analysis and the TechLab E. histolytica-specific antigen detection test. The n...
متن کاملComparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection.
AIMS To assess different laboratory methods for the identification of Entamoeba histolytica in clinical samples. METHODS Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR were compared using 101 stool and pus samples. RESULTS Fifteen of the 101 samples were positive fo...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2005
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.43.5.2168-2172.2005